After optimizing a page for the search engines, I run through my checklist to make sure I have covered the basics. Of course, I may not use all of these on every page, as that would probably make the page look spammy, but I keep them all in a handy checklist to make sure I don't forget anything important.
- keywords in domain name
- keywords in directory and/or filename
- keywords at beginning of title
- keywords in meta description
- keywords in meta keyword
- keywords in header tags - h1, h2
- keywords in bold
- keywords in italic
- keywords underlined
- keywords in list (li)
- keywords in alt tags
- keywords in first sentence of first paragraph
- keywords in middle of text
- keywords at bottom of text